The Voxelation Map of Gene Expression in a Coronal Section of the Mouse Brain

Description or Abstract of the Project: 

Gene expression signatures in the mammalian brain hold the key to understanding neural development and neurological diseases. We have reconstructed two-dimensional images of gene expression for 20,000 genes in a coronal slice of the mouse brain at the level of the striatum by using microarrays in combination with voxelation at a resolution of 1 mm3. Good reliability of the microarray results were confirmed using multiple replicates, subsequent quantitative RT-PCR voxelation, mass spectrometry voxelation, and publicly available in situ hybridization data. Known and novel genes were identified with expression patterns localized to defined substructures within the brain. In addition, genes with unexpected patterns were identified, and cluster analysis identified a set of genes with a gradient of dorsal/ventral expression not restricted to known anatomical boundaries. The genome-scale maps of gene expression obtained using voxelation will be a valuable tool for the neuroscience community.

Principal Investigator (Last Name, First Name): 
Smith, Desmond
Email Address of Principal Investigator: 
dsmith@mednet.ucla.edu
Persons in Charge of Data Collection (Last Name, First Name): 
Smith, Desmond
Email Address of Persons in Charge of Data Collection: 
dsmith@mednet.ucla.edu
Data Registered by (Last Name, First Name): 
Ensberg, Vessela; Shafer, Sharon
Email Address for Person Registering the Data: 
Institution and Department: 
Institution: 
UCLA
Department: 
Molecular and Medical Pharmacol
Date of Collection : 
Sunday, January 13, 2013
Data Collection Methods: 
microarrays, voxelation, mass spectroscopy, RT-PCR
Equipment Used in Data Collection: 
microarray, mass spec, thermocycler
Description of Data Type: 
coronal slices of the mouse brain, microarrays, mass spectra, RT-PCR, images, digital files
Data Format/File Type (example: jpg, xls, txt). Do NOT put periods in the format type or you will get an error message.: 
xls
GPR
Programs Needed to Open the Data: 
Excel
Are These Data Shareable?: 
Yes
Funding Agency (example: NSF, NIH, NOAA, or any other funder): 
UCLA
Keywords : 
S4-S10
2-dimensional images of gene expression for 20 847 genes
PP1r1b
Ndn
Serpinb1a
HSLoH11
Nfix
Pbx3
Gene Bank ID
Date of Registration: 
Wednesday, October 16, 2013